• TECHNOLOGY

OGAB

Ordered Gene Assembly in Bacillus subtilis (OGAB) is a method to assemble DNA fragments through transformation in B. subtilis. First, DNA fragments and assembly plasmid vectors are prepared with 3-4bp overhangs, allowing DNA fragments to be linked together in a specific order and orientation. Recent popular DNA assembly methods in E. coli require in vitro connection products having a circular form, however the efficiency decreases as the assembly scale increases.

B. subtilis transformation does not require circular DNA. Instead, multiple assembly units are inserted as linear DNA that has been prepared in a tandem repeat manner, which is then cleaved and repaired into a circular form through homologous recombination. As it is much easier and less costly to prepare long strands of linear DNA in vitro than circular DNA, this can be leveraged to very efficiently assemble long-chain DNA.

OGAB

Combinatorial-OGAB

Combinatorial libraries of long-chain DNA can be generated in a single reaction by first cutting multiple pre-prepared plasmids with a restriction enzyme, then ligating the mixture utilizing the natural B. subtilis transformation mechanism. Most notably, this method allows for the efficient generation of very large combinatorial libraries of even the most challenging sequences in a very short period of time.

Combinatorial-OGAB

Gene Therapy

Viral vectors, which have been actively researched and developed as gene therapy agents in recent years, are produced by transfection of plasmid DNA, but their successful development as biopharmaceuticals are dependent upon advanced bioprocesses and analytical methods, as well as a high level of expertise.

Synplogen is putting into place robust capabilities for development of viral vectors for therapeutic use, including the necessary bioprocesses and characterization technologies. In addition, Synplogen's synthesis platform can be leveraged to generate previously unobtainable DNA for use in viral vectors.

Gene Therapy

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